Wool Keratin Nanoparticle-Based Micropatterns for Cellular Guidance Applications.

ABSTRACT: The waste stream of low-grade wool is an underutilized source of keratin-rich materials with appropriate methods for upcycling into high value-added products still being an open challenge. In the present work, keratins were precipitated from their water solution to produce hierarchical keratin particles via isoelectric precipitation. Matrix-assisted laser desorption/ionization coupled with time-of-flight tandem mass spectrometry analysis (MALDI-TOF/TOF MS/MS) showed the presence of the amino acid sequence leucine-aspartic acid-valine (LDV) in the extracted keratin. This well-known cell adhesion motif is recognized by the cell adhesion molecule α₄β₁ integrin. We showed that keratin particles had this tripeptide exposed on the surface and that it could be leveraged, via

SUBMITTER: Trojanowska DJ 

PROVIDER: S-EPMC9624257 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature