Spleen Tyrosine Kinase Contributes to Muller Glial Expression of Proangiogenic Cytokines in Diabetes.

ABSTRACT:

Purpose

Neuroglial dysfunction occurs early in the progression of diabetic retinopathy. In response to diabetes or hypoxia, Müller glia secrete cytokines and growth factors that contribute to disease progression. This study was designed to examine common signaling pathways activated in Müller glia by both type 1 and pre-/type 2 diabetes.

Methods

RiboTag (Pdgfra-cre;HA-Rpl22) mice were used to compare the impact of streptozotocin (STZ) and a high-fat, high-sucrose (HFHS) diet on ribosome association of mRNAs in Müller glia by RNA sequencing analysis. Human MIO-M1 Müller cells were exposed to either hyperglycemic or hypoxic culture conditions. Genetic manipulation and pharmacologic inhibition were used to interrogate signaling pathways.

Results

Association of mRNAs enco

SUBMITTER: Yerlikaya EI 

PROVIDER: S-EPMC9624266 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature