Modeling difference x-ray scattering observations from an integral membrane protein within a detergent micelle.

ABSTRACT: Time-resolved x-ray solution scattering (TR-XSS) is a sub-field of structural biology, which observes secondary structural changes in proteins as they evolve along their functional pathways. While the number of distinct conformational states and their rise and decay can be extracted directly from TR-XSS experimental data recorded from light-sensitive systems, structural modeling is more challenging. This step often builds from complementary structural information, including secondary structural changes extracted from crystallographic studies or molecular dynamics simulations. When working with integral membrane proteins, another challenge arises because x-ray scattering from the protein and the surrounding detergent micelle interfere and these effects should be considered during structural

SUBMITTER: Sarabi D 

PROVIDER: S-EPMC9625836 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature