Single-cell RNA sequencing highlights the functional role of human endogenous retroviruses in gallbladder cancer.

ABSTRACT:

Background

Gallbladder cancer (GBC), the most common malignancy of the biliary tract, shows late diagnosis and low survival rate and requires continued search for new diagnostic biomarkers and therapeutic targets. Human endogenous retroviruses (HERVs) are specifically prone to be reactivated in diverse cancers and are implicated in cancer progression and immunotherapy.

Methods

Single-cell RNA sequencing was performed on tumor tissues and paired adjacent tissues from 4 GBC patients. Dual-luciferase reporter assay was applied to measure enhancer activity of HERV sequences.

Findings

We dissected the cellular diversity and described the HERV transcriptomic landscape for GBC. We found that HERVs were transcribed in a cell type-specific manner and different HERV families we

SUBMITTER: Wang J 

PROVIDER: S-EPMC9626538 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature