Blockade of the BLT1-LTB₄ axis does not affect mast cell migration towards advanced atherosclerotic lesions in LDLr^-/- mice.

ABSTRACT: Mast cells have been associated with the progression and destabilization of advanced atherosclerotic plaques. Reducing intraplaque mast cell accumulation upon atherosclerosis progression could be a potent therapeutic strategy to limit plaque destabilization. Leukotriene B₄ (LTB₄) has been reported to induce mast cell chemotaxis in vitro. Here, we examined whether antagonism of the LTB₄-receptor BLT1 could inhibit mast cell accumulation in advanced atherosclerosis. Expression of genes involved in LTB₄ biosynthesis was determined by single-cell RNA sequencing of human atherosclerotic plaques. Subsequently, Western-type diet fed LDLr^-/- mice with pre-existing atherosclerosis were treated with the BLT1-antagonist CP105,696 or vehicle contr

SUBMITTER: Depuydt MAC 

PROVIDER: S-EPMC9626554 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature