Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal.

ABSTRACT: Intestinal organoids, derived from intestinal stem cell self-organization, recapitulate the tissue structures and behaviors of the intestinal epithelium, which hold great potential for the study of developmental biology, disease modeling, and regenerative medicine. The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development. However, the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors. Here, we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells. Furthermore, mechanical stretching stimulates the expansion of SOX9⁺ progenitors

SUBMITTER: Meng F 

PROVIDER: S-EPMC9626719 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature