MiOS, an integrated imaging and computational strategy to model gene folding with nucleosome resolution.

ABSTRACT: The linear sequence of DNA provides invaluable information about genes and their regulatory elements along chromosomes. However, to fully understand gene function and regulation, we need to dissect how genes physically fold in the three-dimensional nuclear space. Here we describe immuno-OligoSTORM, an imaging strategy that reveals the distribution of nucleosomes within specific genes in super-resolution, through the simultaneous visualization of DNA and histones. We combine immuno-OligoSTORM with restraint-based and coarse-grained modeling approaches to integrate super-resolution imaging data with Hi-C contact frequencies and deconvoluted micrococcal nuclease-sequencing information. The resulting method, called Modeling immuno-OligoSTORM, allows quantitative modeling of genes with nucleoso

SUBMITTER: Neguembor MV 

PROVIDER: S-EPMC9627188 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature