A novel severe cerebral venous thrombosis rat model based on semi-ligation combined with ferric chloride and thrombin.

ABSTRACT:

Aims

An applicable cerebral venous sinus thrombosis (CVST) model is imperative for exploring its pathophysiology. We established a novel severe CVST model using semi-ligation, ferric chloride, and thrombin.

Methods

A total of 138 male Sprague-Dawley rats were randomly divided into semi-ligation (n = 75) and non-semi-ligation (n = 63) groups. A sham group (n = 46) was also included. We compared short-term and long-term neurological and cognitive dysfunction, mortality rates, thrombus load, venous infarction volume, the blood-brain barrier permeability, brain water content, and microglia activation among the three groups.

Results

Thrombi involving multiple venous sinuses appeared in all semi-ligation rats within 2 days postoperatively. Compared with the non-semi-ligatio

SUBMITTER: Xiao L 

PROVIDER: S-EPMC9627376 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature