Irradiation enhances the malignancy-promoting behaviors of cancer-associated fibroblasts.

ABSTRACT:

Background

Cancer-associated fibroblasts (CAFs) are the important component of the tumor microenvironment (TME). Previous studies have found that some pro-malignant CAFs participate in the resistance to radiotherapy as well as the initiation and progression of tumor recurrence. However, the exact mechanism of how radiation affects CAFs remains unclear. This study aimed to explore the effect and possible mechanism of radiation-activated CAFs, and its influence on lung cancer.

Methods

CAFs were isolated from surgical specimens in situ and irradiated with 8Gy x-rays. The changes in cell morphology and subcellular structure were observed. CAFs marker proteins such as FAP and α-SMA were detected by Western Blotting. Cell counting kit-8 (CCK8) assay, flow cytometry, wound h

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC9627491 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature