SOX9 maintains human foetal lung tip progenitor state by enhancing WNT and RTK signalling.

ABSTRACT: The balance between self-renewal and differentiation in human foetal lung epithelial progenitors controls the size and function of the adult organ. Moreover, progenitor cell gene regulation networks are employed by both regenerating and malignant lung cells, where modulators of their effects could potentially be of therapeutic value. Details of the molecular networks controlling human lung progenitor self-renewal remain unknown. We performed the first CRISPRi screen in primary human lung organoids to identify transcription factors controlling progenitor self-renewal. We show that SOX9 promotes proliferation of lung progenitors and inhibits precocious airway differentiation. Moreover, by identifying direct transcriptional targets using Targeted DamID, we place SOX9 at the centre of a transc

SUBMITTER: Sun D 

PROVIDER: S-EPMC9627674 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature