Novel target antigens of the variant-specific immune response to Plasmodium falciparum identified by differential screening of an expression library.

ABSTRACT: A primary infection by the Plasmodium falciparum Palo Alto O and R antigenic variants induces a variant-specific immunity in the Saimiri sciureus monkey. We have shown that these variants express distinct PfEMP1 antigens and differ in their levels of expression of additional antigens, including two conserved erythrocyte membrane-associated proteins, HRP1 and PfEMP3. To identify the antigens eliciting a variant-specific response, we conducted a differential screening of a lambdagt11 library with variant-specific sera. We report here the analysis of the 46 anti-R-specific clones. Two specific targets of the anti-R response were identified: (i) PfEMP3, suggesting that immunogenicity of this antigen is modulated by its relative abundance in different variants, and (ii) Asn-rich motifs. Most an

SUBMITTER: Le Scanf C 

PROVIDER: S-EPMC96278 | biostudies-literature | 1999 Jan

REPOSITORIES: biostudies-literature