Apolipoprotein E imbalance in the cerebrospinal fluid of Alzheimer's disease patients.

ABSTRACT:

Objective

The purpose of this study was to examine the levels of cerebrospinal fluid (CSF) apolipoprotein E (apoE) species in Alzheimer's disease (AD) patients.

Methods

We analyzed two CSF cohorts of AD and control individuals expressing different APOE genotypes. Moreover, CSF samples from the TgF344-AD rat model were included. Samples were run in native- and SDS-PAGE under reducing or non-reducing conditions (with or without β-mercaptoethanol). Immunoprecipitation combined with mass spectrometry or western blotting analyses served to assess the identity of apoE complexes.

Results

In TgF344-AD rats expressing a unique apoE variant resembling human apoE4, a ~35-kDa apoE monomer was identified, increasing at 16.5 months compared with wild-types. In humans, apoE isoforms

SUBMITTER: Lennol MP 

PROVIDER: S-EPMC9628034 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature