Isolation and characterization of extracellular vesicles from biotechnologically important fungus Aureobasidium pullulans.

ABSTRACT: Extracellular vesicles (EVs) are increasingly recognized as an important mechanism for cell-cell interactions. Their role in fungi is still poorly understood and they have been isolated from only a handful of species. Here, we isolated and characterized EVs from Aureobasidium pullulans, a biotechnologically important black yeast-like fungus that is increasingly used for biocontrol of phytopathogenic fungi and bacteria. After optimization of the isolation protocol, characterization of EVs from A. pullulans by transmission electron microscopy (TEM) revealed a typical cup-shaped morphology and different subpopulations of EVs. These results were confirmed by nanoparticle tracking analysis (NTA), which revealed that A. pullulans produced 6.1 × 10⁸ nanoparticles per milliliter of cult

SUBMITTER: Cernosa A 

PROVIDER: S-EPMC9628041 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature