Alterations of DNA methylation and expression of genes related to thyroid hormone metabolism in colon epithelium of obese patients.

ABSTRACT:

Background

Colorectal cancer is common among obese individuals. The purpose of the current study was to determine changes in DNA methylation status and mRNA expression of thyroid hormone receptor beta (THRB), as a tumor suppressor, and thyroid hormone inactivating enzyme, type 3 deiodinase (DIO3) genes, in human epithelial colon tissues of healthy obese individuals.

Methods

Colon biopsies were analyzed by methylation sensitive-high resolution melting (MS-HRM) to investigate promoter methylation of DIO3 and THRB, and by quantitative real-time polymerase chain reaction to assay expression of DIO3 and THRB mRNA on eighteen obese and twenty-one normal-weight healthy men.

Results

There was no significant difference in mean methylation levels at the THRB promoter region bet

SUBMITTER: Shimi G 

PROVIDER: S-EPMC9628115 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature