Target-enriched long-read sequencing (TELSeq) contextualizes antimicrobial resistance genes in metagenomes.

ABSTRACT:

Background

Metagenomic data can be used to profile high-importance genes within microbiomes. However, current metagenomic workflows produce data that suffer from low sensitivity and an inability to accurately reconstruct partial or full genomes, particularly those in low abundance. These limitations preclude colocalization analysis, i.e., characterizing the genomic context of genes and functions within a metagenomic sample. Genomic context is especially crucial for functions associated with horizontal gene transfer (HGT) via mobile genetic elements (MGEs), for example antimicrobial resistance (AMR). To overcome this current limitation of metagenomics, we present a method for comprehensive and accurate reconstruction of antimicrobial resistance genes (ARGs) and MGEs from metagenomic

SUBMITTER: Slizovskiy IB 

PROVIDER: S-EPMC9628182 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature