Human platelet lysate-derived extracellular vesicles enhance angiogenesis through miR-126.

ABSTRACT:

Objectives

Extracellular vesicles (EVs) are key biological mediators of several physiological functions within the cell microenvironment. Platelets are the most abundant source of EVs in the blood. Similarly, platelet lysate (PL), the best platelet derivative and angiogenic performer for regenerative purposes, is enriched of EVs, but their role is still too poorly discovered to be suitably exploited. Here, we explored the contribution of the EVs in PL, by investigating the angiogenic features extrapolated from that possessed by PL.

Methods

We tested angiogenic ability and molecular cargo in 3D bioprinted models and by RNA sequencing analysis of PL-derived EVs.

Results

A subset of small vesicles is highly represented in PL. The EVs do not retain aggregation ability, pr

SUBMITTER: Bordin A 

PROVIDER: S-EPMC9628251 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature