MiR-125b-5p modulates the function of regulatory T cells in tumor microenvironment by targeting TNFR2.

ABSTRACT:

Background

Tumor necrosis factor receptor type 2 (TNFR2) is primarily expressed by CD4⁺FoxP3⁺ regulatory T cells (Tregs), especially those present in tumor microenvironment. There is compelling evidence that TNFR2 plays a crucial role in the activation, expansion, and phenotypic stability of Tregs and promotes tumor immune evasion. Understanding of epigenetic regulation of TNFR2 expression in Tregs may help device a novel strategy in cancer immunotherapy.

Methods

MiR-125b-5p-overexpressing or knockdown murine CD4 T cells and Tregs were constructed, and the effect of miR-125b-5p on Tregs proliferation, suppressive function and TNFR2 expression were examined. In vivo antitumor efficacy of Ago-125b-5p (miR-125b-5p agomir) was evaluated in MC38 tumor bearin

SUBMITTER: Jiang M 

PROVIDER: S-EPMC9628696 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature