Rapid Detection of Clostridium tetani by Recombinase Polymerase Amplification Using an Exo Probe.

ABSTRACT: Tetanus is a potentially fatal public health illness resulted from the neurotoxins generated by Clostridium tetani. C. tetani is not easily culturable and culturing the relevant bacteria from infected wounds has rarely been useful in diagnosis; PCR-based assays can only be conducted at highly sophisticated laboratories. Therefore, a real-time recombinase polymerase amplification assay (Exo-RPA) was constructed to identify the fragments of the neurotoxin gene of C. tetani. Primers and the exo probe targeting the conserved region were designed, and the resulting amplicons could be detected in less than 20 min, with a detection limit of 20 copies/reaction. The RPA assay displayed good selectivity, and there were no cross-reactions with other infectious bacteria common in

SUBMITTER: Guo M 

PROVIDER: S-EPMC9628835 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature