Dataset Information

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate.

ABSTRACT: Continued fenvalerate use has caused serious environmental pollution and requires large-scale remediation. Dibutyl phthalate (DBP) was discovered in fenvalerate metabolites degraded by Citrobacter freundii CD-9. Coculturing is an effective method for bioremediation, but few studies have analyzed the degradation pathways and potential mechanisms of cocultures. Here, a DBP-degrading strain (BDBP 071) was isolated from soil contaminated with pyrethroid pesticides (PPs) and identified as Stenotrophomonas acidaminiphila. The optimum conditions for DBP degradation were determined by response surface methodology (RSM) analysis to be 30.9 mg/l DBP concentration, pH 7.5, at a culture temperature of 37.2°C. Under the optimized conditions, approximately 88% of DBP was degraded within 48 h and five metabolites were detected. Coculturing C. freundii CD-9 and S. acidaminiphila BDBP 071 promoted fenvalerate degradation. When CD-9 was cultured for 16 h before adding BDBP 071, the strain inoculation ratio was 5:5 (v/v), fenvalerate concentration was 75.0 mg/l, fenvalerate was degraded to 84.37 ± 1.25%, and DBP level was reduced by 5.21 mg/l. In addition, 12 fenvalerate metabolites were identified and a pathway for fenvalerate degradation by the cocultured strains was proposed. These results provide theoretical data for further exploration of the mechanisms used by this coculture system to degrade fenvalerate and DBP, and also offer a promising method for effective bioremediation of PPs and their related metabolites in polluted environments.

SUBMITTER: Wu M

PROVIDER: S-EPMC9628840 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate.

Wu Min M Tang Jie J Zhou Xuerui X Lei Dan D Zeng Chaoyi C Ye Hong H Cai Ting T Zhang Qing Q

Journal of microbiology and biotechnology 20220201 2

Continued fenvalerate use has caused serious environmental pollution and requires large-scale remediation. Dibutyl phthalate (DBP) was discovered in fenvalerate metabolites degraded by Citrobacter freundii CD-9. Coculturing is an effective method for bioremediation, but few studies have analyzed the degradation pathways and potential mechanisms of cocultures. Here, a DBP-degrading strain (BDBP 071) was isolated from soil contaminated with pyrethroid pesticides (PPs) and identified as S ...[more]

PMID: 35058397

Similar Datasets

Project description:Two bacterial strains, denoted so4 and w15, isolated from wheat straw (WS)-degrading microbial consortia, were found to grow synergistically in media containing WS as the single carbon and energy source. They were identified as Citrobacter freundii so4 and Sphingobacterium multivorum w15 based on 16S rRNA gene sequencing and comparison to the respective C. freundii and S. multivorum type strains. In order to identify the mechanisms driving the synergistic interactions, we analyzed the draft genomes of the two strains and further characterized their metabolic potential. The latter analyses revealed that the strains had largely complementary substrate utilization patterns, with only 22 out of 190 compounds shared. The analyses further indicated C. freundii so4 to primarily consume amino acids and simple sugars, with laminarin as a key exception. In contrast, S. multivorum w15 showed ample capacity to transform complex polysaccharides, including intermediates of starch degradation. Sequence analyses revealed C. freundii so4 to have a genome of 4,883,214 bp, with a G + C content of 52.5%, 4,554 protein-encoding genes and 86 RNA genes. S. multivorum w15 has a genome of 6,678,278 bp, with a G + C content of 39.7%, 5,999 protein-encoding genes and 76 RNA genes. Genes for motility apparatuses (flagella, chemotaxis) were present in the genome of C. freundii so4, but absent from that of S. multivorum w15. In the genome of S. multivorum w15, 348 genes had regions matching CAZy family enzymes and/or carbohydrate-binding modules (CBMs), with 193 glycosyl hydrolase (GH) and 50 CBM domains. Remarkably, 22 domains matched enzymes of glycoside hydrolase family GH43, suggesting a strong investment in the degradation of arabinoxylan. In contrast, 130 CAZy family genes were found in C. freundii so4, with 61 GH and 12 CBM domains identified. Collectively, our results, based on both metabolic potential and genome analyses, revealed the two strains to harbor complementary catabolic armories, with S. multivorum w15 primarily attacking the WS hemicellulose and C. freundii so4 the cellobiose derived from cellulose, next to emerging oligo- or monosaccharides. Finally, C. freundii so4 may secrete secondary metabolites that S. multivorum w15 can consume, and detoxify the system by reducing the levels of (toxic) by-products.

Project description:Dibutyl phthalate (DBP) with teratogenicity, carcinogenesis, and mutagenesis, is a ubiquitous endocrine disruptor in the environment. The widespread usage of plastic mulch has resulted in a severe DBP pollution problem in agricultural soil. One of the most vital ways to mitigate the DBP pollution problem is to use DBP degrading bacteria to reduce the concentration of DBP in agricultural soil. DBP degrading bacteria studied in previous studies mainly come from sewage treatment plants, rivers, landfills, bioreactors, and other environmental media. At the same time, there was relatively little research on DBP degrading bacteria in agricultural soil. Therefore, using the pure culture approach, the candidate DBP degrading strains were isolated and screened from vegetable plots contaminated with plastic mulch in Dayi County, Chengdu City, Sichuan Province, China. The taxonomy of the strains was determined using the 16 sedimentation ribosomal deoxyribonucleic acid (16S rDNA) molecular technique. Furthermore, ultraviolet and visible spectrophotometry and high performance liquid chromatography (HPLC) were used to assess the degrading capability of strains. The findings showed that a total of twenty-six DBP degrading strains were screened from vegetable plots contaminated with plastic mulch in Dayi County, Chengdu City, Sichuan Province, China, and these strains belonged to two phyla: Proteobacteria and Bacillota, eight families, including Pseudomonas, Enterobacteriaceae, and Bacillaceae, as well as ten genera, such as Enterobacter, Pseudomonas, and Bacillus. One of them, the strain SWDB-7 was a potentially new species in the Enterobacter genus. The most prominent genus of the isolated strains was Enterobacter. There were significant variations in the degradation capability of different obtained DBP degrading strains. and their degradation efficiency was 14.12%-91.86%. With a total DBP removal rate of 91.86%, strain SWDB-15 had the most effective degrading capability among them. To sum up, the DBP degrading strains in vegetable plots contaminated with plastic mulch in Dayi County, Chengdu City, Sichuan Province, China are rich in diversity and capable of breaking down DBP.

Dataset Information

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with Citrobacter freundii CD-9 to Degrade Fenvalerate.

Publications

Isolation of Dibutyl Phthalate-Degrading Bacteria and Its Coculture with <i>Citrobacter freundii</i> CD-9 to Degrade Fenvalerate.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets