Detection of monkeypox virus with real-time PCR assays.

ABSTRACT:

Background

Human monkeypox, a zoonotic disease, was first reported outside of Africa during the 2003 US outbreak.

Objectives

We present two real-time PCR assays critical for laboratory diagnosis of monkeypox during the 2003 US outbreak.

Study design

A TaqMan-based assay (E9L-NVAR) targets the orthopoxvirus DNA polymerase gene and detects Eurasian orthopoxviruses other than Variola. A hybridization assay, utilizing a MGB Eclipsetrade mark (Epoch Biosciences) probe, targets an envelope protein gene (B6R) and specifically detects monkeypox virus (MPXV). Assays were validated using coded orthopoxvirus DNA samples and used to evaluate lesion samples from five confirmed US monkeypox cases.

Results

E9L-NVAR did not detect variola (48 strains), North American orthopoxv

SUBMITTER: Li Y 

PROVIDER: S-EPMC9628957 | biostudies-literature | 2006 Jul

REPOSITORIES: biostudies-literature