Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems.

ABSTRACT: In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished.

SUBMITTER: Qi Q 

PROVIDER: S-EPMC9629044 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature