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Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems† † Electronic supplementary information (ESI) available: Extended experimental procedures and figures. See DOI: https://doi.org/10.1039/d2sc02635j


ABSTRACT: In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished. Here, norbornene-tetrazine ligation chemistry is harnessed to control RNA-guided CRISPR systems in vitro and in human cells.

SUBMITTER: Qi Q 

PROVIDER: S-EPMC9629044 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature

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