Notch signaling functions in noncanonical juxtacrine manner in platelets to amplify thrombogenicity.

ABSTRACT:

Background

Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. Existence of functional Notch signaling in enucleate platelets remains elusive.

Methods

Transcripts/peptides of Notch1 and Delta-like ligand (DLL)-4 were detected in platelets isolated from human blood by RT-qPCR, Western analysis and flow cytometry. Platelet aggregation, granule secretion and platelet-leukocyte interaction were analyzed by lumi-aggregometry and flow cytometry. Platelet-derived extracellular vesicles were documented with Nanoparticle Tracking Analyzer. Platelet thrombus on immobilized collagen was quantified using microfluidics platform. Intracellular calcium was monitored by fluorescence spectrophotometry. Whole blood coagulation was studied by thromboelasto

SUBMITTER: Chaurasia SN 

PROVIDER: S-EPMC9629830 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature