Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulations.

ABSTRACT: Tissue-resident macrophages represent a group of highly responsive innate immune cells that acquire diverse functions by polarizing toward distinct subpopulations. The subpopulations of macrophages that reside in skeletal muscle (SKM) and their changes during aging are poorly characterized. By single-cell transcriptomic analysis with unsupervised clustering, we found 11 distinct macrophage clusters in male mouse SKM with enriched gene expression programs linked to reparative, proinflammatory, phagocytic, proliferative, and senescence-associated functions. Using a complementary classification, membrane markers LYVE1 and MHCII identified four macrophage subgroups: LYVE1-/MHCII^hi (M1-like, classically activated), LYVE1+/MHCII^lo (M2-like, alternatively activated), and two

SUBMITTER: Krasniewski LK 

PROVIDER: S-EPMC9629833 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature