Dynamic proteomic and phosphoproteomic atlas of corticostriatal axons in neurodevelopment.

ABSTRACT: Mammalian axonal development begins in embryonic stages and continues postnatally. After birth, axonal proteomic landscape changes rapidly, coordinated by transcription, protein turnover, and post-translational modifications. Comprehensive profiling of axonal proteomes across neurodevelopment is limited, with most studies lacking cell-type and neural circuit specificity, resulting in substantial information loss. We create a Cre-dependent APEX2 reporter mouse line and map cell-type-specific proteome of corticostriatal projections across postnatal development. We synthesize analysis frameworks to define temporal patterns of axonal proteome and phosphoproteome, identifying co-regulated proteins and phosphorylations associated with genetic risk for human brain disorders. We discover proline-d

SUBMITTER: Dumrongprechachan V 

PROVIDER: S-EPMC9629834 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature