Advances in endogenous RNA pull-down: A straightforward dextran sulfate-based method enhancing RNA recovery.

ABSTRACT: Detecting RNA/RNA interactions in the context of a given cellular system is crucial to gain insights into the molecular mechanisms that stand beneath each specific RNA molecule. When it comes to non-protein coding RNA (ncRNAs), and especially to long noncoding RNAs (lncRNAs), the reliability of the RNA purification is dramatically dependent on their abundance. Exogenous methods, in which lncRNAs are in vitro transcribed and incubated with protein extracts or overexpressed by cell transfection, have been extensively used to overcome the problem of abundance. However, although useful to study the contribution of single RNA sub-modules to RNA/protein interactions, these exogenous practices might fail in revealing biologically meaningful contacts occurring in vivo and risk to gen

SUBMITTER: Desideri F 

PROVIDER: S-EPMC9629853 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature