Gene Expression and Immunochemistry Analysis of ADAMTS-1 and Versican in Ameloblastoma.

ABSTRACT:

Background

Ameloblastoma is a benign but locally invasive odontogenic epithelial tumor, associated with a high recurrence rate after treatment. The action of enzymes of the metalloproteinase family is important to the degraded extracellular matrix, contributing to invasion. Thus, this study aimed to investigate the gene and protein expression of ADAMTS-1 and versican in ameloblastoma.

Materials and methods

Twenty cases of ameloblastoma (n = 20) and ten dental follicles (DF) (n = 10) were used as a source for immunochemistry and quantitative RT-PCR for determining the protein and mRNA expressions of the concerned genes, respectively. Moreover, western blot and indirect immunofluorescence analysis were performed in AME cells.

Results

ADAMTS-1 and versican

SUBMITTER: de Souza Neto OR 

PROVIDER: S-EPMC9629950 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature