The structural effect between the output module and chromophore-binding domain is a two-way street via the hairpin extension.

ABSTRACT: Signal transduction typically starts with either ligand binding or cofactor activation, eventually affecting biological activities in the cell. In red light-sensing phytochromes, isomerization of the bilin chromophore results in regulation of the activity of diverse output modules. During this process, several structural elements and chemical events influence signal propagation. In our study, we have studied the full-length bacteriophytochrome from Deinococcus radiodurans as well as a previously generated optogenetic tool where the native histidine kinase output module has been replaced with an adenylate cyclase. We show that the composition of the output module influences the stability of the hairpin extension. The hairpin, often referred as the PHY tongue, is one of the central structura

SUBMITTER: Kurttila M 

PROVIDER: S-EPMC9630206 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature