Instantaneous 4D micro-particle image velocimetry (µPIV) via multifocal microscopy (MUM).

ABSTRACT: Multifocal microscopy (MUM), a technique to capture multiple fields of view (FOVs) from distinct axial planes simultaneously and on one camera, was used to perform micro-particle image velocimetry (µPIV) to reconstruct velocity and shear stress fields imposed by a liquid flowing around a cell. A diffraction based multifocal relay was used to capture images from three different planes with 630 nm axial spacing from which the axial positions of the flow-tracing particles were calculated using the image sharpness metric. It was shown that MUM can achieve an accuracy on the calculated velocity of around (0.52 ± 0.19) µm/s. Using fixed cells, MUM imaged the flow perturbations at sub-cellular level, which showed characteristics similar to those observed in the literature. Using live cells as an

SUBMITTER: Guastamacchia MGR 

PROVIDER: S-EPMC9630545 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature