N6-methyladenosine reader YTHDF3 regulates melanoma metastasis via its 'executor'LOXL3.

ABSTRACT:

Background

A number of studies have demonstrated that N6-methyladenosine (m6A) plays a vital role in the pathological process of various tumours. Recently, it was found that m6A writers or erasers affect the tumourigenesis of melanoma. However, the relationship between m6A readers such as YTH domain family (YTHDF) proteins and melanoma was still elusive.

Methods

RT-qPCR, Western blot and immunohistochemistry were conducted to measure the expression level of YTH N6-methyladenosine RNA binding protein 3 (YTHDF3) and lysyl oxidase-like 3 (LOXL3) in melanoma tissues and cells. The effects of YTHDF3 and LOXL3 on melanoma were verified in vitro and in vivo. Multi-omics analysis including RNA-seq, MeRIP-seq, RIP-seq and mass spectrometry analyses was performed to identify the targe

SUBMITTER: Shi HZ 

PROVIDER: S-EPMC9630608 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature