Extracellular vesicles derived from the choroid plexus trigger the differentiation of neural stem cells.

ABSTRACT: The choroid plexus secrets cerebrospinal fluid (CSF) composed of electrolytes, cytokines, growth factors, metabolites and extracellular vesicles (EVs) that flow through the interconnected brain ventricles. On their course, CSF components can act as signals that affect, for example, neural stem cells (NSCs) residing in niches of the ventricular wall. We studied EV-born CSF signals in an in vitro culture system. We purified EVs from the secretome of a choroid plexus cell line (Z310 cells), and from primary choroid plexus cultures and co-cultured those EVs with NSCs isolated from the niche of the lateral and the third ventricle. EVs^Z310 and EVs^CHP were purified by differential centrifugation. This yielded fractions of EVs of 50-150-nm diameter that induced a complex mult

SUBMITTER: Ditte Z 

PROVIDER: S-EPMC9630752 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature