Human in vitro neurocardiac coculture (ivNCC) assay development for evaluating cardiac contractility modulation.

ABSTRACT: Two of the most prominent organ systems, the nervous and the cardiovascular systems, are intricately connected to maintain homeostasis in mammals. Recent years have shown tremendous efforts toward therapeutic modulation of cardiac contractility and electrophysiology by electrical stimulation. Neuronal innervation and cardiac ganglia regulation are often overlooked when developing in vitro models for cardiac devices, but it is likely that peripheral nervous system plays a role in the clinical effects. We developed an in vitro neurocardiac coculture (ivNCC) model system to study cardiac and neuronal interplay using human induced pluripotent stem cell (hiPSC) technology. We demonstrated significant expression and colocalization of cardiac markers including troponin, α-actinin, and neuronal ma

SUBMITTER: Narkar A 

PROVIDER: S-EPMC9630755 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature