A critical developmental window for ELAV/Hu-dependent mRNA signatures at the onset of neuronal differentiation.

ABSTRACT: Cell-type-specific gene regulatory programs are essential for cell differentiation and function. In animal neurons, the highly conserved ELAV/Hu family of proteins promotes alternative splicing and polyadenylation of mRNA precursors to create unique neuronal transcript isoforms. Here, we assess transcriptome profiles and neurogenesis success in Drosophila models engineered to express differing levels of ELAV activity in the course of development. We show that the ELAV-mediated establishment of a subset of neuronal mRNA isoforms at the onset of neuron differentiation constitutes a developmental bottleneck that cannot be overcome later by the nuclear activation of the paralog found in neurons (FNE). Loss of ELAV function outside of that critical time window results in neurological defects. W

SUBMITTER: Carrasco J 

PROVIDER: S-EPMC9631114 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature