Metabolic reprogramming under hypoxic storage preserves faster oxygen unloading from stored red blood cells.

ABSTRACT: Stored red blood cells (RBCs) incur biochemical and morphological changes, collectively termed the storage lesion. Functionally, the storage lesion manifests as slower oxygen unloading from RBCs, which may compromise the efficacy of transfusions where the clinical imperative is to rapidly boost oxygen delivery to tissues. Recent analysis of large real-world data linked longer storage with increased recipient mortality. Biochemical rejuvenation with a formulation of adenosine, inosine, and pyruvate can restore gas-handling properties, but its implementation is impractical for most clinical scenarios. We tested whether storage under hypoxia, previously shown to slow biochemical degradation, also preserves gas-handling properties of RBCs. A microfluidic chamber, designed to rapidly switch bet

SUBMITTER: Rabcuka J 

PROVIDER: S-EPMC9631703 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature