Electrochemical Carbamazepine Aptasensor for Therapeutic Drug Monitoring at the Point of Care.

ABSTRACT: Monitoring the anti-epileptic drug carbamazepine (CBZ) is crucial for proper dosing, optimizing a patient's clinical outcome, and managing their medication regimen. Due to its narrow therapeutic window and concentration-related toxicity, CBZ is prescribed and monitored in a highly personalized manner. We report an electrochemical conformation-changing aptasensor with two assay formats: a 30 min assay for routine monitoring and a 5 min assay for rapid emergency testing. To enable "sample-to-answer" testing, a de novo CBZ aptamer (K _d < 12 nM) with conformational switching due to a G-quadruplex motif was labeled with methylene blue and immobilized on a gold electrode. The electrode fabrication and detection conditions were optimized using electrochemical techniques and visu

SUBMITTER: Chung S 

PROVIDER: S-EPMC9631757 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature