NRAS^Q61R mutation in human endothelial cells causes vascular malformations.

ABSTRACT: Somatic mutations in NRAS drive the pathogenesis of melanoma and other cancers but their role in vascular anomalies and specifically human endothelial cells is unclear. The goals of this study were to determine whether the somatic-activating NRAS^Q61R mutation in human endothelial cells induces abnormal angiogenesis and to develop in vitro and in vivo models to identify disease-causing pathways and test inhibitors. Here, we used mutant NRAS^Q61R and wild-type NRAS (NRAS^WT) expressing human endothelial cells in in vitro and in vivo angiogenesis models. These studies demonstrated that expression of NRAS^Q61R in human endothelial cells caused a shift to an abnormal spindle-shaped morphology, increased proliferation, and migration. NRAS^Q61R e

SUBMITTER: Boscolo E 

PROVIDER: S-EPMC9631803 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature