Identification of essential genes in Mycobacterium avium subsp. paratuberculosis genome for persistence in dairy calves.

ABSTRACT: To cause disease Mycobacterium avium subsp. paratuberculosis needs to enter mammalian cells, arrest phagosomal maturation and manipulate the host immune system. The genetic basis of the bacterial capacity to achieve these outcomes remains largely unknown. Identifying these genes would allow us to gain a deeper understanding of MAP's pathogenesis and potentially develop a live attenuated Johne's disease vaccine by knocking out these genes. MAP genes demonstrated to be essential for colonization in the natural host, ruminants, are unknown. Genome-wide transposon mutagenesis and high-throughput sequencing were combined to evaluate the essentiality of each coding region in the bacterial genome to survive in dairy calves. A saturated library of 3,852 MAP Tn mutants, with insertion

SUBMITTER: Eshraghisamani R 

PROVIDER: S-EPMC9631821 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature