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Simple fluorescence chemosensor for the detection of calcium ions in water samples and its application in bio-imaging of cancer cells† † Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2ra04815a

ABSTRACT: This article describes the design, synthesis and characterization of a sensor suitable for practical measurement of ionized calcium in water samples and cancer cells. Calcium is an important ion in living organs and works as a messenger in several cellular functions. A lack of Ca ions interrupts the immune system and can lead to several diseases. A novel magnetic-polydopamine nanoparticle (PDNP)/rhodamine B (RhB)/folic acid (FA) nanoparticle was developed for the determination of calcium ions in MCF 7 cell lysates and water samples. Furthermore, the produced nanoparticle was employed for bioimaging of folate receptor (FR)-overexpressed cancer cells. This nanoprobe displayed a bright photoluminescence emission at 576 nm under an excitation wavelength of 420 nm. In the presence of calcium ions, the fluorescence emission of the MNPs-PDNPs/RhB/FA probe was proportionally decreased from 20 ng mL−1 to 100 ng mL−1 and 0.5 μg mL−1 to 20 μg mL−1 with a lower limit of quantification (LLOQ) of about 20 ng mL−1. The developed sensor showed a low-interference manner in the presence of possible coexistence interfering ions. In addition, this nanomaterial showed excellent biocompatibility with favorable differentiation ability to attach to the FR-positive cancer cells. The MNPs-PDNPs/RhB/FA nanoparticle has been utilized for bioimaging of the MCF 7 cell with favorable differentiation ability. Synthesis of the MNPs-PDNPs/RhB/FA probe and application for bioimaging and Ca detection.

SUBMITTER: Salek-Maghsoodi M

PROVIDER: S-EPMC9631868 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature

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