Identification and Quality Evaluation of Velvet Antler by DNA Barcoding and Stable Isotope Techniques Combined with Chemometrics.

ABSTRACT: This study aimed to identify Velvet antler and its counterfeits and to further evaluate their quality. Mitochondrial cytochrome b (Cytb) was used as a target gene to identify Velvet antler samples, and a DNA barcoding method was established for species origin identification in Velvet antlers. After identification, the stable isotope contents and ratios were adopted to evaluate the quality of different specifications of authentic Velvet antler in combination with chemometrics. Two stable isotope contents (C % and N %) and ratios (δ¹³C and δ¹⁵N) in three kinds of Velvet antler slices of different specifications, namely, wax slices, powder slices, and bone slices, were determined. Nine Velvet antler samples sold in the market were identified for label conformity.

SUBMITTER: Zeng B 

PROVIDER: S-EPMC9631900 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature