CRISPR screens in Drosophila cells identify Vsg as a Tc toxin receptor.

ABSTRACT: Entomopathogenic nematodes are widely used as biopesticides^1,2. Their insecticidal activity depends on symbiotic bacteria such as Photorhabdus luminescens, which produces toxin complex (Tc) toxins as major virulence factors^3-6. No protein receptors are known for any Tc toxins, which limits our understanding of their specificity and pathogenesis. Here we use genome-wide CRISPR-Cas9-mediated knockout screening in Drosophila melanogaster S2R+ cells and identify Visgun (Vsg) as a receptor for an archetypal P. luminescens Tc toxin (pTc). The toxin recognizes the extracellular O-glycosylated mucin-like domain of Vsg that contains high-density repeats of proline, threonine and serine (HD-PTS). Vsg orthologues in mosquitoes and beetles contain HD-PTS and can function as pTc r

SUBMITTER: Xu Y 

PROVIDER: S-EPMC9631961 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature