Coemergence of the Amphipathic Helix on Ameloblastin With Mammalian Prismatic Enamel.

ABSTRACT: To investigate correlation between the ameloblastin (Ambn) amino acid sequence and the emergence of prismatic enamel, a notable event in the evolution of ectodermal hard tissues, we analyzed Ambn sequences of 53 species for which enamel microstructures have been previously reported. We found that a potential amphipathic helix (AH) within the sequence encoded by Exon 5 of Ambn appeared in species with prismatic enamel, with a few exceptions. We studied this correlation by investigating synthetic peptides from different species. A blue shift in fluorescence spectroscopy suggested that the peptides derived from mammalian Ambn interacted with liposomes. A downward shift at 222 nm in circular dichroism spectroscopy of the peptides in the presence of liposomes suggested that the peptides of mamm

SUBMITTER: Su J 

PROVIDER: S-EPMC9631975 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature