Segregationally stabilised plasmids improve production of commodity chemicals in glucose-limited continuous fermentation.

ABSTRACT:

Background

The production of chemicals via bio-based routes is held back by limited easy-to-use stabilisation systems. A wide range of plasmid stabilisation mechanisms can be found in the literature, however, how these mechanisms effect genetic stability and how host strains still revert to non-productive variants is poorly understood at the single-cell level. This phenomenon can generate difficulties in production-scale bioreactors as different populations of productive and non-productive cells can arise. To understand how to prevent non-productive strains from arising, it is vital to understand strain behaviour at a single-cell level. The persistence of genes located on plasmid vectors is dependent on numerous factors but can be broadly separated into structural stability and seg

SUBMITTER: Allen JR 

PROVIDER: S-EPMC9632041 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature