IL-6 production through repression of UBASH3A gene via epigenetic dysregulation of super-enhancer in CD4⁺ T cells in rheumatoid arthritis.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is associated with immune dysfunction. UBASH3A as a negative regulator of T cell receptors (TCRs) signaling is a susceptible factor in RA. The aim of this study was to determine the role of UBASH3A in RA pathogenesis, by assessing the role of super-enhancer (SE) in the control of UBASH3A expression in CD4⁺ T cells and the contribution of the latter in proinflammatory cytokine production in patients with RA.

Methods

UBASH3A mRNA and protein levels were quantified by PCR and western blotting, respectively. The cells were treated with a locked nucleic acid to inhibit enhancer RNA (eRNA) expression. Chromatin immunoprecipitation was used to identify the factors recruited to UBASH3A loci displaying SE architecture. CD4⁺ T

SUBMITTER: Yamagata K 

PROVIDER: S-EPMC9632101 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature