Establishing an experimental model for canine atopic dermatitis through epicutaneous application of Dermatophagoides farinae.

ABSTRACT: There is no established protocol for the development of an experimental canine atopic dermatitis model in laboratory beagles. This study aimed to establish an experimental model that mimics spontaneous canine atopic dermatitis (CAD) clinically, immunologically, and microbiologically, by repeated epicutaneous applications of mite antigens and to describe the entire process including sensitization and provocation in detail for reproducibility. Six intact male laboratory beagle dogs aged 14 months were included in this study. During the sensitization and provocation phase, the house dust mite (HDM) paste consisted of Dermatophagoides farinae (Der f ) and mineral oil, which was applied focally to the 10 × 10 cm area of the right groin as evenly as possible. Further, 120 mg of

SUBMITTER: Kim SW 

PROVIDER: S-EPMC9632614 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature