Basal extrusion of single-oncogenic mutant cells induces dome-like structures with altered microenvironments.

ABSTRACT: At the initial stage of carcinogenesis, oncogenic transformation occurs in single cells within epithelial layers. However, the behavior and fate of the newly emerging transformed cells remain enigmatic. Here, using originally established mouse models, we investigate the fate of RasV12-transformed cells that appear in a mosaic manner within epithelial tissues. In the lung bronchial epithelium, most majority of RasV12-transformed cells are apically extruded, whereas noneliminated RasV12 cells are often basally delaminated leading to various noncell-autonomous changes in surrounding environments; macrophages and activated fibroblasts are accumulated, and normal epithelial cells overlying RasV12 cells overproliferate and form a convex multilayer, which is termed a 'dome-like structure'. In add

SUBMITTER: Shirai T 

PROVIDER: S-EPMC9633292 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature