In vivo phenotyping of the microvasculature in necrotizing enterocolitis with multicontrast optical imaging.

ABSTRACT:

Objective

Necrotizing enterocolitis (NEC) is the most prevalent gastrointestinal emergency in premature infants and is characterized by a dysfunctional gut microcirculation. Therefore, there is a dire need for in vivo methods to characterize NEC-induced changes in the structure and function of the gut microcirculation, that is, its vascular phenotype. Since in vivo gut imaging methods are often slow and employ a single-contrast mechanism, we developed a rapid multicontrast imaging technique and a novel analyses pipeline for phenotyping the gut microcirculation.

Methods

Using an experimental NEC model, we acquired in vivo images of the gut microvasculature and blood flow over a 5000 × 7000 μm² field of view at 5 μm resolution via the following two endogenous contra

SUBMITTER: Senarathna J 

PROVIDER: S-EPMC9633336 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature