Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues.

ABSTRACT: Extracellular vesicles (EVs) are nanoscale vesicles secreted into the extracellular space by all cell types, including neurons and astrocytes in the brain. EVs play pivotal roles in physiological and pathophysiological processes such as waste removal, cell-to-cell communication and transport of either protective or pathogenic material into the extracellular space. Here we describe a detailed protocol for the reliable and consistent isolation of EVs from both murine and human brains, intended for anyone with basic laboratory experience and performed in a total time of 27 h. The method includes a mild extracellular matrix digestion of the brain tissue, a series of filtration and centrifugation steps to purify EVs and an iodixanol-based high-resolution density step gradient that fractionates

SUBMITTER: D'Acunzo P 

PROVIDER: S-EPMC9633367 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature