Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyte.

ABSTRACT: In heart failure, an increased abundance of post-translationally detyrosinated microtubules stiffens the cardiomyocyte and impedes its contractile function. Detyrosination promotes interactions between microtubules, desmin intermediate filaments, and the sarcomere to increase cytoskeletal stiffness, yet the mechanism by which this occurs is unknown. We hypothesized that detyrosination may regulate the growth and shrinkage of dynamic microtubules to facilitate interactions with desmin and the sarcomere. Through a combination of biochemical assays and direct observation of growing microtubule plus-ends in adult cardiomyocytes, we find that desmin is required to stabilize growing microtubules at the level of the sarcomere Z-disk, where desmin also rescues shrinking microtubules from continued

SUBMITTER: Salomon AK 

PROVIDER: S-EPMC9633452 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature