Bile acids regulate the epithelial Na⁺ channel in native tissues through direct binding at multiple sites.

ABSTRACT: Bile acids, originally known to emulsify dietary lipids, are now established signalling molecules that regulate physiological processes. Signalling targets several proteins that include the ion channels involved in regulating intestinal motility and bile viscosity. Studies show that bile acids regulate the epithelial sodium channel (ENaC) in cultured cell models and heterologous expression systems. ENaC plays both local and systemic roles in regulating extracellular fluids. Here we investigated whether bile acids regulate ENaC expressed in native tissues. We found that taurocholic acid and taurohyodeoxycholic acid regulated ENaC in both the distal nephron and distal colon. We also tested the hypothesis that regulation occurs through direct binding. Using photoaffinity labelling, we found e

SUBMITTER: Wang XP 

PROVIDER: S-EPMC9633555 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature