Effects of Some Interferon-Related Proteins on Influenza A Viruse RNA Polymerase Activity.

ABSTRACT:

Objectives

Interferons (IFNs) are one of the most important components of innate immunity against viruses, especially those carrying the RNA genomes such as influenza viruses. Upon viral infection, the IFNs are rapidly secreted, inducing the expression of several genes in the target cells and establishing an antiviral state. In this study, the effects of proteins encoded by some IFN-related genes on influenza A virus RNA-dependent RNA polymerase enzyme were investigated. We evaluated the importance of these proteins in the pathogenesis of different influenza A virus types.

Materials and methods

The IFN-related genes were amplified by polymerase chain reaction from the HEK293 cDNA library and cloned into pCHA expression vector. The expression of genes and subcellular localiza

SUBMITTER: Caglayan E 

PROVIDER: S-EPMC9634453 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature