Postmitotic centriole disengagement and maturation leads to centrosome amplification in polyploid trophoblast giant cells.

ABSTRACT: DNA replication is normally coupled with centriole duplication in the cell cycle. Trophoblast giant cells (TGCs) of the placenta undergo endocycles resulting in polyploidy but their centriole state is not known. We used a cell culture model for TGC differentiation to examine centriole and centrosome number and properties. Before differentiation, trophoblast stem cells (TSCs) have either two centrioles before duplication or four centrioles after. We find that the average nuclear area increases approximately eight-fold over differentiation, but most TGCs do not have more than four centrioles. However, these centrioles become disengaged, acquire centrosome proteins, and can nucleate microtubules. In addition, some TGCs undergo further duplication and disengagement of centrioles, resulting in

SUBMITTER: Buss G 

PROVIDER: S-EPMC9634975 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature